Fig. 1: O + C induces immunogenic lung cancer cell death. | Cell Death & Disease

Fig. 1: O + C induces immunogenic lung cancer cell death.

From: Ischemia and reperfusion injury combined with cisplatin induces immunogenic cell death in lung cancer cells

Fig. 1

A–C LLC and A549 cells were treated with Dimethyl fumarate (DMF) solvent control, OGD/R, CDDP or O + C at the indicated dose for 20 h and then stained with an anti-annexin-V fluorescein isothiocyanate (FITC)-conjugated antibody and propidium iodine (PI) before a subsequent analysis by flow cytometry A, B or by TUNEL staining C. D, E PBS (n = 6), F/T LLC cells (n = 7) and LLC cells treated with1 μM MTX (n = 7), 100 μM CDDP (n = 6), or O + C (n = 6), for 20 h and incubated for 4 h without the chemotherapeutic drugs were inoculated into immunocompetent C57BL/6 mice, which were rechallenged 7 days later with live LLC cells D. The tumor growth curves E and overall survival F of C57BL/6 mice after injected with different tumor vaccine are shown. The quantification in A combines data from three independent experiments. The quantification in B combines data from four independent experiments. Data are reported as the mean ± SEM, and statistical analyses were performed with a two-tailed unpaired Student’s t test. The tumor incidence in the vaccination experiments was analyzed by means of the log-rank test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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