Fig. 3: O + C-treated LLC cells are phagocytized and induce bone marrow-derived dendritic cell maturation. | Cell Death & Disease

Fig. 3: O + C-treated LLC cells are phagocytized and induce bone marrow-derived dendritic cell maturation.

From: Ischemia and reperfusion injury combined with cisplatin induces immunogenic cell death in lung cancer cells

Fig. 3

A Representative confocal images show the uptake of DiI-labeled cellular material by DiO-labeled BMDCs. B Representative flow cytometry dot plots show the uptake of DiI-labeled cellular material by BMDCs. Untreated, CDDP-treated, OGD/R-treated and O + C-treated cells were incubated with the BMDCs for 4 h. The phagocytosis assay shows effective uptake of the O + C-treated LLC cells by the BMDCs. C–F Representative dot plots show the gating and percentages of CD11c + MHC-II + CD80 + , CD11c + MHC-II + CD86 + and CD11c + CD80 + CD86 + BMDCs that were cocultured at a ratio of 1:4 with OGD/R-treated cells, CDDP-treated cells, O + C-treated cells, or F/T-treated necrotic LLC cells for 20 h. LPS was used as a positive control. The quantification of B combines data from three independent experiments, and the quantification of E to F combines data from six independent experiments. Data are reported as the mean ± SEM, and statistical analyses were performed with one-way ANOVA followed by the Dunnett post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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