Fig. 5: I + C inhibits tumor growth in immunocompetent mice.
A Laser speckle contrast imaging was used to assess the inguinal tumor blood supply before and during I/R. B Laser signal values of the ROI are graphically presented and show the progression from pre-ischemia through reperfusion of the blood supply. Data were recorded every 2 min after clamping the superficial abdominal artery. C A total of 5 × 105 cells were inoculated subcutaneously into the right groin of C57BL/6 or Balb/c-nu mice. On days 8 and 10, the right superficial abdominal artery of the mice was blocked for 90 min and then allowed to reperfuse. On days 8, 10 and 13, 3 mg/kg CDDP was injected intraperitoneally. N = 7 for each group. On day 18, the mice were sacrificed, the tumor and spleen were collected for further testing. D, E The tumor growth curve D and plot of the tumor weight E of the C57BL/6 mice after treated with sham surgery, I/R, CDDP, or I + C are shown. F Representative images of TUNEL staining of tumors in C57bl/C mice treated as in A. G, H The tumor growth curve G and plot of the tumor weight H of athymic nude mouse Balb/c-nu after being treated as in D and E are shown. N = 7 for each group. I Mice were treated as in C, after reperfusion for 10 min, 30 min, 2 h, and 4 h, the tumor were removed and proteins were detected by western blot. Data are reported as the mean ± SEM, and statistical analyses were performed with unpaired Student’s t test E, H, one-way ANOVA followed by the Dunnett post hoc test I, and two-way ANOVA D. *P < 0.05, **P < 0.01 and ****P < 0.0001.
