Fig. 7: GAL3 inhibition abolishes the hepatoprotection in fibrotic mice against acute insult by upregulating pyroptosis rather than necroptosis signalling.

a The protein expression levels of the necroptosis signalling molecule p-MLKL and pyroptosis signalling molecules, including NLRP3, GSDMD, ASC and caspase-1, as detected by western blot in the livers of fibrotic mice upon insult with or without GB1107 treatment. b The mRNA expression levels of necroptosis signalling molecules, including Ripk1 and Mlkl, and pyroptosis signalling molecules, including Nlrp3, Asc, Caspase1 and Il18, in the livers of fibrotic mice upon insult with or without GB1107 treatment (n = 5–8). Group comparisons were performed using Student’s t test or the Mann–Whitney U test.