Fig. 1: Identification and characterization of hsa_circ_0007367 in PDAC. | Cell Death & Disease

Fig. 1: Identification and characterization of hsa_circ_0007367 in PDAC.

From: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression

Fig. 1

A Hierarchical clustering of differentially expressed circRNAs in 3 pairs of PDAC and their matched normal tissues. Red represents upregulated circRNAs, and green represents downregulated circRNAs. B Volcano plot of differentially expressed circRNAs, the −log10 (p-value) and the log2 (fold change) are plotted on the y and x axes, respectively. The red dots represent the differentially expressed circRNAs with statistical significance. C The scatter plot shows the dysregulated circRNAs in PDAC tissues compared with matched normal pancreas tissues. The space above the top green line and below the bottom green line indicated more than 1.5-fold change. D The heatmap for 15 differentially up- and downregulated circRNAs. E qRT-PCR analysis of the five dysregulated circRNAs in PANC-1 cells compared to HPDE cells (the normal pancreatic cell line). F, G qRT-PCR for the expression of hsa_circ_0007367 in PDAC cell lines and tissues. H Schematic illustration of the genomic location and splicing pattern of hsa_circ_0007367, with the splicing site identified by Sanger sequencing. I FISH assay showed the subcellular localization of hsa_circ_0007367 in PDAC cells. All data are shown as the mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01.

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