Fig. 5: SEAS1 inhibits TNBC progression by targeting miR-3940-3p.

A RT-qPCR results show the expression levels of miR-3940-3p in the MDA-MB-231 cells co-transfected with siRNA against SEAS1 and the miR-3940-3p inhibitor. B Spearman correlation analysis results show the relationship between miR-3940-3p and SEAS1 expression levels in 50 breast tumor tissues. C–E CCK8, colony formation, and EdU assay results show the proliferation rates of MDA-MB-231 cells co-transfected with siRNA against SEAS1 and the miR-3940-3p inhibitor. F FACS analysis results show the apoptotic rates in the MDA-MB-231 cells co-transfected with siRNA against SEAS1 and the miR-3940-3p inhibitor and the corresponding controls. G Transwell assay results show the migration and invasion rates of MDA-MB-231 cells co-transfected with siRNA against SEAS1 and miR-3940-3p inhibitor and the corresponding controls. H Western blot assay shows the levels of caspase 3, caspase 7, BAX, PARP, and Bcl2 proteins in the MDA-MB-231 cells co-transfected with siRNA against SEAS1 and the miR-3940-3p inhibitor and the corresponding controls. I Western blot analysis shows the levels of EMT-related proteins namely, N-cadherin, Vimentin, Snail, and E-cadherin in MDA-MB-231 cells co-transfected with siRNA against SEAS1 and the miR-3940-3p inhibitor and the corresponding controls. *P < 0.05, **P < 0.01, and ***P < 0.001. Representative data from at least 3 experiments with comparable results are shown.