Fig. 2: LINC00239 upregulates colorectal cancer cell proliferation by inhibiting ferroptosis. | Cell Death & Disease

Fig. 2: LINC00239 upregulates colorectal cancer cell proliferation by inhibiting ferroptosis.

From: Long noncoding RNA LINC00239 inhibits ferroptosis in colorectal cancer by binding to Keap1 to stabilize Nrf2

Fig. 2

A RNA-seq-based heatmap indicating the changes of ferroptosis-related genes in SW620 cells knockout LINC00239. B KEGG analysis of LINC00239-related gene co-expression networks from the sequencing data. C GSEA analysis of LINC00239-related gene co-expression networks from the sequencing data. D–H GSH/GSSG ratios (D), ROS levels (E), Lipid ROS (F), cell viability (G), and Fe2+ concentration (H) was measured in the four indicated cell lines after treated with 10 μM erastin for 48 h and the addition of 2 µM ferrostatin-1 (Fer-1). I, J Colony-formation and CCK-8 assay to evaluate the cell viability of LINC00239 on colorectal cancer cells after treated with 2 μM erastin and the addition of 2 µM ferrostatin-1 (Fer-1). K, L Spheroids generated from the indicated cell lines were cultured for 96 h and treated with 15 μM erastin for 48 h. Dead cells were stained by SYTOX Green (original magnification, ×40). Data shown represent mean ± SD from three independent experiments. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test.

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