Fig. 5: A1 deletion worsens retinal thinning, apoptosis, horizontal cells loss, and glial activation after OIR. | Cell Death & Disease

Fig. 5: A1 deletion worsens retinal thinning, apoptosis, horizontal cells loss, and glial activation after OIR.

From: Targeting proliferative retinopathy: Arginase 1 limits vitreoretinal neovascularization and promotes angiogenic repair

Fig. 5

A–D SD-OCT analysis of 12-week-old mice retinas showed a significant reduction in thickness of the total retina, ganglion cell complex (GCC), and outer nuclear layer plus inner segments (ONL + IS) in the A1 KO OIR retinas as compared to WT OIR retinas. E, F TUNEL labeling and quantification at P9 showed a significant increase in TUNEL-positive cells in A1 KO OIR retina sections as compared to WT OIR retinas. Scale bar = 50 µm. G, H Calbindin labeling and quantitation of retina sections at P17 showed horizontal cell loss after OIR that was further aggravated in A1 KO OIR retinas. Scale bar = 50 µm. I–K GFAP immunolabeling of retina cross-sections at P17 showed increased expression of GFAP in Müller cells beyond their end-feet after OIR, which was further increased in A1 KO retinas. Western blotting on P17 retina homogenates and quantification confirmed the immunolabeling results. Scale bar = 50 µm.

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