Fig. 7: Endothelial but not myeloid A1 deletion decreases pathological angiogenesis in OIR. | Cell Death & Disease

Fig. 7: Endothelial but not myeloid A1 deletion decreases pathological angiogenesis in OIR.

From: Targeting proliferative retinopathy: Arginase 1 limits vitreoretinal neovascularization and promotes angiogenic repair

Fig. 7

A–C Analysis of P17 OIR retinas or using lectin staining showed a significant decrease in pathological neovascularization (NV, highlighted in white) in endothelial A1 knockout mice (E-A1−/−) as compared to littermate floxed controls (A1f/f), while the AVA (yellow outline) showed a decreasing trend that was not statistically significant. Scale bar = 100 µm. D–F Analysis of myeloid A1 knockout mice (M-A1−/−) OIR retinas showed no change in AVA or NV tuft formation at P17 as compared to littermate floxed controls (A1f/f). Scale bar = 100 µm. G, H Choroidal angiogenesis assay and quantification showed marked suppression of the angiogenic response with endothelial A1 deletion or PEG-A1 treatment. I–L Treatment of bovine retinal endothelial cells with PEG-A1 for 6 h led to increased ERK phosphorylation as measured by western blotting while 24 h treatment increased FGF2 protein levels.

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