Fig. 4: The activation of the IGF1/IGF1R-related signaling pathways by GASP1 in breast cancer cells.

a The potential downstream pathways of GASP1 were identified by FunRich. b The effect of GASP1 overexpression on the activities of multiple signaling pathways, including IGF1/IGF1R1, NF-κB, PI3K/AKT, and MAPK/ERK pathways. GAPDH was used as a loading control. c The effect of GASP1 knockout on the activities of IGF1/IGF1R1, NF-κB, PI3K/AKT, and MAPK/ERK pathways. d Representative tumor sections from GASP1-knockout and control mice were subjected to IHC staining with the indicated antibodies. Scale bar, 200 µm. Data were presented as mean ± SD. **P < 0.01; ***P < 0.001.