Fig. 3: KCTD9 inhibits CRC cell proliferation and metastasis in vitro.
From: KCTD9 inhibits the Wnt/β-catenin pathway by decreasing the level of β-catenin in colorectal cancer
A Western blotting analysis of the CRC cell lines LoVo and SW620 after infection with lentiviral particles based on control pCDH or pCDH-KCTD9 vectors to overexpress KCTD9. β-actin was used as a loading control. Data shown represent three independent experiments (top) and relative density were quantified by using ImageJ software (bottom). B Assessment of cell viability in the cells from A measured using CCK-8 assays. C Assessment of proliferation in the cells from A measured using Count Star (Countstar BioTech) over 1–4 days by cell counting. D, E Assessment of migration in the cells from A measured in wound healing assays. Representative images at time 0 and 48 h (scale bar=200 µm) (D) with quantitation of wound closure (E). D represent three independent experiments. F, G Assessment of migration and invasion in the cells from A using Transwell™ assays. Representative images of migrating/invading cells after 24 h (scale bar =200 µm) (F) with comparative quantitation of total migrating cells (G). F Represent three independent experiments. A, B, E, G Are mean ± SD, n = 3 independent experiments, two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001. C is mean ± SD, n = 3 independent experiments, two-way ANOVA with Bonferroni’s multiple comparison post-test, **p < 0.01.
