Fig. 4: FIIN-2 induced autophagic flux in A549 and A549/DDP cells through mTOR inhibition and the activation of the class III PI3K pathway. | Cell Death & Disease

Fig. 4: FIIN-2 induced autophagic flux in A549 and A549/DDP cells through mTOR inhibition and the activation of the class III PI3K pathway.

From: Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma

Fig. 4

a A549 and A549/DDP cells were treated with 10 μmol/L FIIN-2 for 24 h, and the autophagy characteristics were observed by transmission electron microscopy (TEM). b The MDC assay was used to observe the induction of autophagy by FIIN-2 (0–20 μmol/L) in A549 and A549/DDP cells. c Cells were treated with FIIN-2 (0–20 μmol/L) for 24 h, and the levels of Parkin, Beclin-1, p62, and LC3 were detected by western blot analysis. d A549 and A549/DDP cells were cotreated with 10 μmol/L CQ and 10 μmol/L FIIN-2 for 24 h, then the levels of LC3-II/LC3-I and p62 were detected by western blot. e After the mRFP-GFP-LC3 lentiviral vector was transfected into A549 and A549/DDP cells, the effect of FIIN-2 (10 μmol/L) on autophagic flux was evaluated (yellow dots: autophagosomes; red dots: autophagolysosomes). f Cells were treated with FIIN-2 (0–20 μmol/L) for 24 h, and the levels of p-mTOR and Vps34 were detected by western blot analysis. g The effect of FIIN-2 (10 μmol/L) on the Vps34-Beclin-1 complex was analysed by immunoprecipitation. β-actin and β-tubulin were used as the loading controls. Each bar corresponds to the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001.

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