Fig. 5: Inhibition of autophagy enhanced FIIN-2-mediated cytotoxicity in A549 and A549/DDP cells. | Cell Death & Disease

Fig. 5: Inhibition of autophagy enhanced FIIN-2-mediated cytotoxicity in A549 and A549/DDP cells.

From: Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma

Fig. 5

a Cells were treated with FIIN-2 (10 μmol/L) for 48 h, and the levels of Bcl-2, Bax, cleaved Caspase-3, LC3, and p62 at different times were detected by western blot analysis. Cells were treated with FIIN-2 (10 μmol/L), CQ (10 μmol/L), or 3-MA (5 mmol/L) alone and FIIN-2 combined with CQ/3-MA for 24 h. b Cell viability was detected by CCK-8 assay. c The expression of Bax and cleaved Caspase-3 was detected by western blot analysis. β-actin was used as a loading control. Each bar corresponds to the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001. CF-, cleaved Caspase-3.

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