Fig. 4: FOXD1 activates the transcription of SLC2A1 and HOXA11-AS. | Cell Death & Disease

Fig. 4: FOXD1 activates the transcription of SLC2A1 and HOXA11-AS.

From: FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis

Fig. 4

A The intersection between RNA-Seq and ChIP-Seq in FOXD1 overexpression PANC-1 cell and control cells, represented as a Venn diagram. B Relative HOXA11-AS and SLC2A1 expression of MIA PaCa-2 and PANC-1 cells with FOXD1 overexpression and knockdown were detected by RT-qPCR. C FOXD1 binding motif predicted by JASPAR. D Schematic diagram of potential FOXD1 binding sites in the promoter region of HOXA11-AS and SLC2A1. E Schematic diagram of primers designed for regions of HOXA11-AS and SLC2A1 promoter. F, G ChIP-PCR analysis of enrichment of FOXD1 on HOXA11-AS or SLC2A1 promoter. IgG was used as a negative control. H, I Schematic diagram of dual-luciferase reporter vector construction for HOXA11-AS or SLC2A1 promoter. J, K The luciferase activity of the wild-type and mutant HOXA11-As or SLC2A1 promoters with indicated cells. *p < 0.05; **p < 0.01.

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