Fig. 5: HOXA11-AS upregulates SLC2A1 by sponging miR-148b-3p. | Cell Death & Disease

Fig. 5: HOXA11-AS upregulates SLC2A1 by sponging miR-148b-3p.

From: FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis

Fig. 5

A HOXA11-AS and SLC2A1 were detected with RT-qPCR when HOXA11-AS was inhibited. B, C Subcellular localization of HOXA11-AS was detected by FISH or nucleocytoplasmic fractionation RT-qPCR. D Potential miRNAs regulated by HOXA11-AS which can bind with SLC2A1 were forecasted with indicated websites. E Predicted miRNAs were detected by RT-qPCR when HOXA11-AS was inhibited. F miR-148b-3p and SLC2A1 were detected with RT-qPCR when miR-148b-3p was overexpressed. G Predicted binding sites between HOXA11-AS and miR-148b-3p. H Luciferase activities were detected when cells co-transfected with miR-148b-3p and 3′UTR of HOXA11-AS. I Predicted binding sites between miR-148b-3p and SLC2A1. J Luciferase activities were detected when cells co-transfected with miR-148b-3p and 3′UTR of SLC2A1. K RIP assay was conducted to confirm the interaction between HOXA11-AS, miR-148b-3p, and SLC2A1. L Correlations between HOXA11-AS, miR-148b-3p, and SLC2A1 expression in 40 clinical samples was detected by RT-qPCR. *p < 0.05; **p < 0.01.

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