Fig. 1: Isolation and characterisation of non-myogenic mesenchymal cells. | Cell Death & Disease

Fig. 1: Isolation and characterisation of non-myogenic mesenchymal cells.

From: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients

Fig. 1

A Schematic representation of non-myogenic mesenchymal cell isolation protocol (panel created with Biorender.com). B Gating strategy for non-myogenic mesenchymal cell sorting from the CD56− cell fraction. C Representative histograms of specific markers in the CD56− cell fraction. D Heatmap showing the relative expression levels of positive markers. Original values are ln(x)-transformed. Rows are centred and scaled. Rows are clustered using Euclidean distance and Ward linkage. 9 rows, 13 columns. E PCA analysis by singular value decomposition of the same dataset showed in D. X and Y axis show principal component 1 (PC1) and principal component 2 (PC2). The percentage of the total variance explained by each PC is indicated. F Loadings (i.e. contributions) of each variable to PC2. G–J Relative expression, expressed as median fluorescence intensity (MFI) of CD201, CD140a, CD73 and CD90.

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