Fig. 1: ASF1A was upregulated in CML-BC patients and involved in cell differentiation. | Cell Death & Disease

Fig. 1: ASF1A was upregulated in CML-BC patients and involved in cell differentiation.

From: Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling

Fig. 1

a Relative expression of ASF1A mRNA in CML-CP and CML-BP patients using the GEO database (GSE4170). b qRT-PCR analysis of ASF1A mRNA levels in CML-CP and CML-BP patients. c ICC analysis of ASF1A protein levels. Scale bar, 200 μm. d–f qRT-PCR analysis of ASF1A mRNA levels in K562 and MEG01 cells pretreated with solvent (control) or DMSO/ATRA/PMA. g Western blot analysis of ASF1A in K562 and MEG01 cells pretreated with solvent (control) or DMSO/ATRA/PMA. Statistical significance was determined by Student’s t-test. Data are shown as mean ± standard deviation (SD). Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections in d–g. *P < 0.05, **P < 0.01, ***P < 0.001.

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