Fig. 4: ASF1A promoted Notch signaling activation. | Cell Death & Disease

Fig. 4: ASF1A promoted Notch signaling activation.

From: Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling

Fig. 4

a, b qRT-PCR analysis of ASF1A, c-Myc, and HES1 mRNA levels in K562 cells expressing empty vector (shNC) or shASF1A-1/shASF1A-2. c, d qRT-PCR analysis of ASF1A, c-Myc, and HES1 mRNA levels in MEG01 cells expressing empty vector (shNC) or shASF1A-1/shASF1A-2. e, f qRT-PCR analysis of ASF1A, c-Myc, and HES1 mRNA levels in K562 and MEG01 cells expressing either empty vector (NC) or ASF1A. g Western blot analysis of ASF1A, c-Myc, and HES1 in K562 and MEG01 cells transfected as indicated. Statistical significance was determined by Student’s t test. Data are shown as mean ± standard deviation (SD). Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections in a–g. *P < 0.05, **P < 0.01, ***P < 0.001. h Western blot analysis of ASF1A, c-Myc, and HES1 in K562 and MEG01 expressing either empty vector (NC) or ASF1A, treated with control, IMR-1 (20 μM) or FLI-06 (5 μM) for 48 h. Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections and then treated with the compounds in h.

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