Fig. 5: ASF1A cooperates with RBPJ activates Notch signaling. | Cell Death & Disease

Fig. 5: ASF1A cooperates with RBPJ activates Notch signaling.

From: Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling

Fig. 5

a Co-immunoprecipitation analysis of the interaction between ASF1A and RBPJ in K562 and MEG01 cells. b–e Enrichment of ASF1A and RBPJ at c-Myc (b, d) and HES1 (c, e) promoters in K562 and MEG01 cells. f–i RBPJ enrichment at c-Myc (f, h) and HES1 (g, i) promoters in K562 and MEG01 cells expressing empty vector (shNC) or shASF1A-1. Histone H3 and IgG antibodies were used as positive and negative controls, respectively. j–m Luciferase activity analysis of c-Myc (j, l) and HES1 (k, m) activation in K562 and MEG01 cells transfected with empty vector (shNC) or shASF1A-1. Luciferase activity was determined 48 hours after transfection, and normalized according to Renilla luciferase activity. P values in b–e were determined by one-way ANOVA, and P values in f–m were calculated using Student’s t test. Data are shown as mean ± standard deviation (SD). Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections in b–m. *P < 0.05, **P < 0.01, ***P < 0.001.

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