Fig. 6: ASF1A licenses H3K56ac modification in the promoter regions of Notch target genes. | Cell Death & Disease

Fig. 6: ASF1A licenses H3K56ac modification in the promoter regions of Notch target genes.

From: Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling

Fig. 6

a IF staining of histone H3K56 acetylation in K562 and MEG01 cells expressing empty vector (shNC) or shASF1A-1. b–e Enrichment of H3K56ac at c-Myc (b, d) and HES1 (c, e) promoters in K562 and MEG01 cells expressing empty vector (shNC) or shASF1A-1. Histone H3 and IgG antibodies were used as positive and negative controls, respectively. Statistical significance was determined by Student’s t test. Data are shown as mean ± standard deviation (SD). Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections in a–e. *P < 0.05, **P < 0.01.

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