Fig. 7: ASF1A promotes the development of CML in vivo. | Cell Death & Disease

Fig. 7: ASF1A promotes the development of CML in vivo.

From: Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling

Fig. 7

a–h NOD-SCID mice were injected subcutaneously with K562 cells expressing empty vector (shNC) or shASF1A-1. The protein level of H3K56Ac were analyzed by IHC (a, b). The mRNA and protein levels of c-Myc (c, e), HES1 (d, f), CD13 (g), and CD61 (h) were analyzed by qRT-PCR and IHC. β2-M expression was used for normalization. i–k Primary tumor gross appearance (i), tumor growth curve (j), and tumor weight analysis (k) of NOD-SCID mice injected subcutaneously with K562 cells expressing empty vector (shNC) or shASF1A-1. Statistical significance was determined by Student’s t test. Data are shown as mean ± standard deviation (SD). Data are presented as the mean ± SD of five biologically independent animals in a, b. Data are shown as a representative result with three repeats from three independent experiments. The cell lines are applied with three independent lentiviral infections and then treated with the compounds in c–h. Data are presented as the mean ± SD of six biologically independent animals in i–k. *P < 0.05, **P < 0.01, ***P < 0.001.

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