Fig. 1: Ablation of Hnrnpk results in the occurrence of dwarfism and abnormal growth plate development. | Cell Death & Disease

Fig. 1: Ablation of Hnrnpk results in the occurrence of dwarfism and abnormal growth plate development.

From: Hnrnpk maintains chondrocytes survival and function during growth plate development via regulating Hif1α-glycolysis axis

Fig. 1

A Schematic diagram of the strategy constructing HnrnpkloxP mice. B General inspection and skeletal preparation of E18.5 WT and CKO embryos (left panel) and quantified comparison of limbs length and vertebrae height between E18.5 WT and CKO embryos (right panel). n = 3 biological replicates. Scale bar: 1 mm. C H&E staining (left panel) and Safranine O staining (right panel) of tibia of E18.5 WT and CKO embryos. Black arrows: atypical chondrocytes in resting and proliferating zones. Scale bar: 100 μm. D Quantification of the height of proliferating zone and hypertrophic zones according to H&E staining of tibia of E18.5 WT and CKO embryos. n = 5 biological replicates. E H&E staining of lumbar vertebrae of E18.5 WT and CKO embryos. Red boxes: magnification of vertebral growth plate. Black dotted lines: range of ossification center. Scale bar: 100 μm. F, G von Kassa staining (F) and Toluidine blue staining (G) of tibia of E18.5 WT and CKO embryos. Scale bar: 100 μm. p-value was calculated by two-tailed unpaired Student’s t-test. Data were shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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