Fig. 5: circ_CEA downregulation suppresses tumor growth and promotes apoptosis in vivo. | Cell Death & Disease

Fig. 5: circ_CEA downregulation suppresses tumor growth and promotes apoptosis in vivo.

From: Circ_CEA promotes the interaction between the p53 and cyclin-dependent kinases 1 as a scaffold to inhibit the apoptosis of gastric cancer

Fig. 5

A photos of subcutaneous tumors. After subcutaneous injection of AGS cells, BALB/c nude mice were randomly divided into four groups, and administrated with siNC (control group), circ_CEA simix, Dox+siNC (Dox group), and circ_CEA simix + Dox, respectively. B The tumor sizes were measured twice a week. *P < 0.05 vs control group, #P < 0.05 vs circ_CEA simix group. C The expression level of circ_CEA in subcutaneous tumors was evaluated by qRT-PCR assay. D Paraffin sections were prepared from the subcutaneous tumors, and TUNEL assay were performed to evaluate apoptosis. E–I The expression levels of p53 target genes (NOXA, Puma, Fas, and Bax) and FoxO3 target gene, Bim in the subcutaneous tumors were determined by qRT-PCR assay. J IHC of c-PARP and Bax in the xenograft tumors from nude mice. *P < 0.05 vs control group; #P < 0.05 vs circ_CEA simix group; &P < 0.05 vs Dox group.

Back to article page