Fig. 6: CA3 inhibits ADM, SOX9 expression, and PAF1/YAP1 co-expression.
From: PAF1 cooperates with YAP1 in metaplastic ducts to promote pancreatic cancer
A Light microscope images of pancreatic acinar cell explants from KC mice embedded in collagen treated with vehicle control or CA3 for 72 hr. The bar graph shows the number of duct-like structures per field. Data are mean ± SD, n = 3. Significance was determined with a student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, ns: non-significant, p > 0.05. B, C Confocal images of whole-mount immunofluorescence staining on the acinar explants embedded in collagen treated with vehicle control or CA3. Explants in collagen discs were stained with FITC-labeled Dolichos biflorus agglutinin (DBA), a duct-specific lectin, and rhodamine-labeled Peanut agglutinin (PNA), an acinar-specific lectin, and antibodies for PAF1, YAP1, and SOX9.
