Fig. 2: Pontin facilitates GBM cell proliferation and survival.

a Western blot of Pontin in the extracts of the established Pontin-silenced or -overexpressed U87MG and U251 cells. Loading control, GAPDH. b Representative images of EdU staining (left) and the quantitative analysis of EdU-positive rates among the multiple groups (right). c Cell survival results reflected by colony formation assay. Data in b, c are expressed as means ± SDs, n = 3. ***P < 0.001 by one-way ANOVA (Dunnett’s post test).