Fig. 4: Global profiles of Pontin-regulated differential gene expression and cellular signalling in GBM cells.

a Differential expressed coding genes affected by Pontin knockdown screened by RNA-seq (FC > 2, FDR < 0.05) were used to make the Volcano plot. b Downregulated genes incorporated in a were analyzed using STRING to plot the protein-protein association network with respective function annotation. c GSEA plots suggested a prominent correlation between Pontin and cellular signalling. d qRT-PCR confirmation of Pontin-regulated expression of genes involved in several biological processes or cellular signalling in indicated U87MG cells. Data are expressed as means ± SDs, n = 3.