Fig. 6: Pontin interacts with LEF1 to activate TGFBR2 gene transcription in GBM cells. | Cell Death & Disease

Fig. 6: Pontin interacts with LEF1 to activate TGFBR2 gene transcription in GBM cells.

From: Recruitment of LEF1 by Pontin chromatin modifier amplifies TGFBR2 transcription and activates TGFβ/SMAD signalling during gliomagenesis

Fig. 6

a Venn plot showed the overlapping of Pontin interacting proteins with possible transcription factors regulating TGFBR2 gene expression. SF splicing factors, TF transcription factors. b Representative images showing that Pontin (red) colocalized with LEF1 (green) in U87MG and U251 cell nuclei. Scale bar, 10 μm. c Co-IP confirmation of the interaction between Pontin (flag-tagged) and endogenous LEF1 (upper) or HA-tagged LEF1 (bottom) in U87MG cells. d Effect of Pontin or LEF1 on the luciferase transcriptional response of the reporter driven by TGFBR2 promoter (−2000 to 0). e LEF1-binding sites predicted in the TGFBR2 promoter (left) and ChIP-qPCR analyses of the capacities for them to bind with LEF1 (right). Data in d, e are expressed as means ± SDs, n = 3.

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