Fig. 7: TGFβR2 efficiently mediates the GBM malignancy caused by Pontin. | Cell Death & Disease

Fig. 7: TGFβR2 efficiently mediates the GBM malignancy caused by Pontin.

From: Recruitment of LEF1 by Pontin chromatin modifier amplifies TGFBR2 transcription and activates TGFβ/SMAD signalling during gliomagenesis

Fig. 7

a Western blot analyses of Pontin, TGFβR2, p-SMAD2/3, SMAD2/3 and CTGF in the established TGFβR2-restored U87MG and U251 cells as indicated. b qRT-PCR detection of TGFβ/SMAD target gene expression in the established TGFβR2-restored U87MG cells. c The rescue effects of TGFβR2 on GBM cell proliferation (EdU staining, left), survival (colony formation, middle) and invasion (transwell invasion, right). Representative images from triple experiments (upper) and the quantification results (bottom) are shown. Data in b, c are expressed as means ± SDs, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA (Dunnett’s post test).

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