Fig. 4: Decrease of miR-216a-5p is involved in ML323-mediated DR5 mRNA upregulation. | Cell Death & Disease

Fig. 4: Decrease of miR-216a-5p is involved in ML323-mediated DR5 mRNA upregulation.

From: Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5

Fig. 4

A Investigation of microRNAs in Caki-1 cells treated to 30 μM ML323 for 24 h. B Caki-1 cells were transfected with miR-negative control (NC), miR-7-5p mimic, miR-221-3p mimic, miR-216a-5p mimic, or miR-21-3p mimic followed by 30 μM ML323 for 24 h. C, D Caki-1 cells were transfected with miR-NC or miR-216a-5p mimic followed by 30 μM ML323 for 24 h. DR5 protein and mRNA expression were analyzed using western blotting (C) and qPCR (D). E Caki-1 cells were transfected with luciferase reporter gene for DR5 3′UTR WT or mutant followed by 30 μM ML323 for 24 h and analyzed using luciferase activity assay. F Caki-1 and A549 cells were transfected with miR-216 promoter plasmid followed by 30 μM ML323 for 12 h. Protein expression was measured by western blotting (B, C). Values in the graphs (A, D–F) represent the mean ± SD of three independent experiments. *P < 0.01 compared to the control. #P < 0.01 compared to ML323 treated miR-NC-transfected group. **P < 0.01 compared to miR-216a-5 mimic-treated DR5 3′UTR WT.

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