Fig. 3: TIPE1 decreases osteosarcoma cell migration, invasion and metastasis.

A Wound healing assay showed that overexpression of TIPE1 inhibited wound closure of MNNG/HOS Cl #5 cells. B Knocking down TIPE1 expression strongly improved wound closure in MG63 cells. C Transwell assays showed that cell migration and invasion were decreased in MNNG/HOS Cl #5 cells transfected with TIPE1, but these abilities were enhanced by knocking down TIPE1 expression in MG63 cells. D TIPE1 overexpression attenuated tumor metastasis in vivo as shown by bioluminescence images (n = 3 mice/group). ***P < 0.001, **P < 0.01. The data represent the means ± SDs of 3 replicates.