Fig. 7: TIPE1 reduces STAT3 protein expression in osteosarcoma. | Cell Death & Disease

Fig. 7: TIPE1 reduces STAT3 protein expression in osteosarcoma.

From: TIPE1 inhibits osteosarcoma tumorigenesis and progression by regulating PRMT1 mediated STAT3 arginine methylation

Fig. 7

A Effect of TIPE1 on the PRMT1-mediated transactivation of STAT3. Increasing amounts of PRMT1 together with STAT3 or TIPE1 were transfected into 293T cells. After transfection, the cells were treated with IL-6 (10 ng/ml) for 30 min. The luciferase activity of the APRE reporter gene was determined. PRMT1(VLD-AAA) as a negative control. B TIPE1 full length (FL) and M1 truncation, but not M2 reduced STAT3 protein expression by using Western blotting method. C, D STAT3 abrogates PRMT1 rescued the inhibition of osteosarcoma cell proliferation that caused by TIPE1 by using CCK8 method. (E) Analysis of tumor tissues from nude mice showed that the overexpression of TIPE1 decreased the expression of STAT3 as shown by immunohistochemical staining. F Analysis of 20 osteosarcoma examples showed that TIPE1 expression has a negative relationship with STAT3 expression, as assessed by immunohistochemistry assay. G Schematic diagram illustrating the mechanism by which TIPE1 inhibits osteosarcoma progression. The data represent the means ± SDs of 3 replicates.

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