Fig. 3: Inhibition of circRNF144B reduced OC cell proliferation and mobility, as well as increased autophagy. | Cell Death & Disease

Fig. 3: Inhibition of circRNF144B reduced OC cell proliferation and mobility, as well as increased autophagy.

From: CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1

Fig. 3

A CCK-8 results indicated that circRNF144B knockdown inhibited the cell viability of SKOV3 and OVCAR-3. B Colony formation assay indicated that circRNF144B knockdown inhibited cell colony formation in SKOV3 and OVCAR-3 cells. C EDU positive rate was reduced in SKOV3 and OVCAR-3 cells with circRNF144B knockdown. Scale bar, 100 μm. D Wound healing assay was used to detect the effects of circRNF144B knockdown on SKOV3 and OVCAR-3 cell migration. Scale bar, 50 μm. E Transwell assay was used to detect the effects of circRNF144B knockdown on SKOV3 and OVCAR-3 cell invasion. Scale bar, 50 μm. F–H The volume and weight of tumor tissues derived from SKOV3 cells with circRNF144B knockdown and control SKOV3 cells. I-J Number of metastatic foci in the lung tissues from the mice injected with SKOV3 cells with circRNF144B knockdown and control SKOV3 cells. K Western blot was used to detect the expression of LC3 and P62 in SKOV3 and OVCAR-3 cells after circRNF144B knockdown. L Confocal microscopy was used to detect the number of autophagosomes and autophagolysosomes in SKOV3 cell after circRNF144B knockdown. Scale bar, 25 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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