Fig. 4: MiR-342-3p was identified as a target miRNA of circRNF144B, and played as a suppressor in OC. | Cell Death & Disease

Fig. 4: MiR-342-3p was identified as a target miRNA of circRNF144B, and played as a suppressor in OC.

From: CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1

Fig. 4

A Two online databases including CircBank and Starbase were used to predict the target miRNAs of circRNF144B. B Expression of seven potential target miRNAs was detected by qRT-PCR in SKOV3 and OVCAR-3 cells with circRNF144B-overexpression. C Pearson correlation analysis for circRNF144B and miR-342-3p in OC tissues. D The binding site between circRNF144B and miR-342-3p. E Double luciferase reporting assays indicated the direct binding between circRNF144B and miR-342-3p. F CCK-8 was used to determine the cell viability of SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. G Colony formation assay was used to determine colony formation in SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. H EDU-positive rates were detected in SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. Scale bar, 100 μm. I–J Wound healing and transwell assays were used to detect the migration and invasion of SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. Scale bar, 50 μm.*, P < 0.05; **, P < 0.01.

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