Fig. 5: FBXL11 was identified as a target mRNA of miR-342-3p, and played as an oncogene in OC. | Cell Death & Disease

Fig. 5: FBXL11 was identified as a target mRNA of miR-342-3p, and played as an oncogene in OC.

From: CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1

Fig. 5

A FBXL11 was determined as the target mRNA of miR-342-3p according to the flow chart. B Binding site between miR-342-3p and FBXL11 was exhibited. C Double luciferase reporting assays indicated the direct binding between FBXL11 and miR-342-3p. D qRT-PCR was used to detect the mRNA levels of FBXL11 in SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. E Western blot was used to detect the protein levels of FBXL11 in SKOV3 and OVCAR-3 cells after miR-342-3p-knockdown and miR-342-3p-overexpression. F-G IHC was used to detect the expression levels of FBXL11 in OC tissues with different stage. G qRT-PCR were used to detect the expression levels of FBXL11 in OC tissues and adjacent tissues. H Pearson correlation analysis for FBXL11 and miR-342-3p in OC tissues. I CCK-8 assay was used to determine viability of SKOV3 and OVCAR-3 cells after FBXL11 knockdown. J Colony formation assay was used to detect colony formation in SKOV3 and OVCAR-3 cells after FBXL11 knockdown. K EDU positive rate in SKOV3 and OVCAR-3 cells after FBXL11 knockdown was detected. Scale bar, 100 μm. L-M Wound healing assay and transwell assay was used to detect the migration and invasion of SKOV3 and OVCAR-3 cells after FBXL11 knockdown. Scale bar, 50 μm. *, P < 0.05; **, P < 0.01.

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