Fig. 6: Increased Nitric oxide induces S-nitrosylation of CSF1R and augments the action of CSF1R inhibition to suppress PCa in vitro.
From: S-nitrosylation of CSF1 receptor increases the efficacy of CSF1R blockage against prostate cancer
A S-nitrosylation in tumor lysates in mice xenografts treated with vehicle (Control) and S-nitrosoglutathione (GSNO) for n = 3 samples. B A 3D structure of CSF1R showing 3 cysteine sites highlighted in yellow with the highest threshold for nitrosylation. C A topology map of CSF1R showing intracellular and extracellular domains and the location of cysteine sites. D Representative images show sub-cellular localization of wild-type and three CSF1R mutants (M1, M2, and M3) with deletions at C224, C278, and C830 cysteine residues in 22Rv1 cells. E S-nitrosylation in cell lysates in 22Rv1 cell transfected with wild-type mutants with deletion at sites C224, C278, and C830 in the presence/absence of 50 μm S-nitrosoglutathione (GSNO). F Cytokine array image of the 22Rv1 cell lysates under different experimental conditions-untreated control, cells treated with CSF1R inhibitor at 0.5 μm dose, and cells transfected with all 3 mutants along with the CSF1Ri. G Heat map showing different cytokines differentially expressed in cell lysates treated with CSF1Ri and CSF1R mutants with CSF1Ri. Data are presented as mean ± SEM. ***P < 0.001; **P < 0.01.
