Fig. 1: TRIM11 regulates KDM5C stability.
From: Regulation of KDM5C stability and enhancer reprogramming in breast cancer
A Lentiviruses containing phage-HA-TRIM11 or phage-HA-KDM5Cwere prepared and used to infect MDA-MB-231 cells. Stable cell lines were selected by puromycin. Western blotting was performed as indicated. B 293T cells were transfected with indicated plasmids. After 24 h, cells were treated with DMSO, MG132 (10 μM for 12 h), CQ (20 μM for 9 h), NH4Cl (20 mM for 9 h), 3-MA (5 mM for 9 h), and harvested for western blot. C 293T cells were transfected with indicated plasmids for 36 h followed by treatment with 10 μM MG132 for 12 h, and harvested for western blot. D 293T cells were transfected with indicated plasmids and treated with 100 μg/ml CHX for indicated time. Cells were harvested for western blot. HA-KDM5C abundance was quantified by ImageJ and plotted as indicated. E, F TRIM11 was knocked down in MDA-MB-231 (E) or MDA-MB-468 (F) cells with siRNAs. After 48 h, cells were harvested for western blot as indicated. G TRIM11 was knocked down by siRNA in MDA-MB-231 cells, and 48 h later cells were treated with 100 μg/ml CHX for indicated time. Cells were harvested for western blot. KDM5C abundance was quantified by ImageJ and plotted as indicated.
