Fig. 3: TRIM11 promotes proliferation and migration of breast cancer cells via degrading KDM5C.

A TRIM11 knockdown (TRIM11-KD), KDM5C knockdown (KDM5C-KD) and Double knockdown (DKD) MDA-MB-231 cell lines were constructed using CRISPR/Cas9 system. Two sgRNAs were designed per gene. B Cell proliferation of the cells in (A) were monitored with real-time cell analysis (RTCA). C,D Cell migration of the above cell lines were tested by transwell assay. Four views of the cell migration images were taken for each cell line. The number of moving cells was counted by image J. Bar = 200 μm. E The indicated stable cells derived from MDA-MB-231 were injected into nude mice. The tumors were dissected and pictured. F Tumor growth curve (left) and tumor weight (right) were shown as mean ± SEM. ^*p value ≤ 0.05, ^**p value ≤ 0.01, ^***p value ≤ 0.001. G Two tumors from each group was random picked and assayed with western blotting to confirm they are from original cell lines.