Fig. 7: PDI interacts with PHB2 to attenuate autophagic activity. | Cell Death & Disease

Fig. 7: PDI interacts with PHB2 to attenuate autophagic activity.

From: Protein disulfide isomerase blocks the interaction of LC3II-PHB2 and promotes mTOR signaling to regulate autophagy and radio/chemo-sensitivity

Fig. 7

A–C HCT116 cells were transfected with 1–3 µg PDI-Flag vector (A). Immunoprecipitation of PDI by anti-PDI was performed to determine the competitiveness of the interaction (B). and the statistical data are shown in (C). D Schematic diagram of PDI mutant model. E HCT116 cells were transfected with PDI (Vector), PDI (WT), PDI (P1), PDI (P2), and PDI (P3) and stimulated with IR (4 Gy). The lysates were subjected to immunoprecipitation with an anti-Flag antibody. The precipitates were analyzed by immunoblotting with PDI, PHB2, Flag, LC3, and ACTIN. F, G CCK-8 assay analysis of the impact of PDI (Vector), PDI (WT), PDI (P1), PDI (P2), and PDI (P3) on the viability of HCT116 cells after stimulation with IR (4 Gy) or cisplatin (20 μM). Data were pooled from three independent experiments and the results are represented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.

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