Fig. 3: MSC-Exos attenuate glycolysis in tubular epithelial cells. | Cell Death & Disease

Fig. 3: MSC-Exos attenuate glycolysis in tubular epithelial cells.

From: Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM

Fig. 3

MSC-Exos (100 µg) or PBS was administered intravenously on Day 0 after UUO induction. Kidneys were isolated from sham, UUO and MSC-Exo-treated mice on Day 7. TCMK-1 cells were incubated with TGF-β1 with or without MSC-Exos (20 µg). Cells and cell supernatants were collected at 48 h. A The expression of HK2 and PFKFB3 in renal tissues was measured by western blot analysis (n = 3 mice per group). B The lactate concentration of kidney tissue homogenates was quantified by a lactate assay kit (n = 5 mice per group). C Immunohistochemistry staining of HK2 in kidney tissues (n = 5 mice per group; scale bar=100 μm). Representative images from one experiment out of three are shown. D Protein levels of HK2 and PFKFB3 in TCMK-1 cells were assessed by western blot analysis (n = 3). E Lactate concentration in the supernatant of TCMK-1 cells was assayed by a lactate assay kit (n = 4). F Real-time ECAR of PBS-, TGF-β1-, and MSC-Exo-treated TCMK-1 cells was determined by a Seahorse XF96 Extracellular Flux Analyzer (n = 6). Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001.

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