Fig. 3: PLEKHH2 knockdown inhibits lung cancer cell growth and invasion. | Cell Death & Disease

Fig. 3: PLEKHH2 knockdown inhibits lung cancer cell growth and invasion.

From: PLEKHH2 binds β-arrestin1 through its FERM domain, activates FAK/PI3K/AKT phosphorylation, and promotes the malignant phenotype of non-small cell lung cancer

Fig. 3

All three PLEKHH2-siRNAs down-regulated endogenous PLEKHH2 expression. A Cell lines transfected with PLEKHH2-siRNA plasmid showed decreased PLEKHH2 expression. Clone formation assay (B) and CCK-8 assay (C) showed that PLEKHH2-siRNA treatment led to a decrease in cell proliferation. The Scratch wound assay (D), Transwell migration assay (E), Matrigel Transwell assay (F), and 3D invasion experiment (G) showed that PLEKHH2 knockdown inhibited the biological functions of lung cancer cells, including migration and invasion. Western blotting showed that downregulation of PLEKHH2 decreased the expression of proliferation- and invasion-related proteins, including MMP2, MMP9, CDK4, CDK6, CyclinD1, CyclinE, RhoA, RhoC, and Cdc42 (H). The other two siRNAs showed similar changes, as shown in Supplementary Fig. 1 and Supplementary Fig. 2. P < 0.05 indicates statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001.

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