Fig. 4: Functional validation of lineage-selective SEs. | Cell Death & Disease

Fig. 4: Functional validation of lineage-selective SEs.

From: Lineage-selective super enhancers mediate core regulatory circuitry during adipogenic and osteogenic differentiation of human mesenchymal stem cells

Fig. 4

A, B RT-qPCR analysis of gene expression normalized to GAPDH expression level. The results were presented as means ± S.D. *P < 0.05, **P < 0.01. C Representative images show ALP staining in hMSCs with or without knockout the FR of osteoblasts-selective SE that targeting MEF2A. D RT-qPCR analysis of gene expression normalized to ACTIN expression level. ns: not significant. E Representative images show nile red staining in hMSCs after 7 days of adipocytic differentiation with or without knockout the FR of MEF2A osteoblasts-selective SE. Images were captured and fluorescence intensity was quantitated using Image J software. The percentage fluorescence intensity relative to control is shown and expressed as mean ± S.D. (n = 3). Scare bar: 100 μm. ns: not significant. F, G RT-qPCR analysis of gene expression normalized to ACTIN expression level. *P < 0.05, **P < 0.01, ***P < 0.001. H Representative images showed nile red staining in hMSCs after 7 days of adipocytic differentiation with or without the FR of NFIL3 ADI-lineage SE. Images were captured and fluorescence intensity was quantitated using Image J software. The percentage fluorescence intensity relative to control is shown and expressed as mean ± S.D. (n = 3). Scare bar: 100 μm. *P < 0.05. I RT-qPCR analysis of gene expression normalized to GAPDH expression level. ns: not significant. J Representative images show ALP staining in hMSCs with or without knockout the FR of osteoblasts-selective SE that targeting NFIL3.

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