Fig. 2: CASZ1 regulates sympathoadrenal lineage genes.

A GSEA shows a positive enrichment of axonogenesis, neurotransmitter transport, and catecholamine secretion genes upon induction of CASZ1b in SY5Y cells (top panel). GSEA shows a positive enrichment of axonogenesis, neurotransmitter transport and neurotrophin signaling genes when CASZ1b is overexpressed in AS cells. B The single-cell mRNA expression pattern of Casz1, CRC components, and sympathoadrenal lineage-determination and mark genes in E12.5 mouse embryonic adrenal medulla were analyzed using the Harvard interactive interface tools (http://pklab.med.harvard.edu/cgi-bin/R/rook/nc.SS2_16_250-2/index.html; scRNAseq results are available at Gene Expression Omnibus, GSE99933). Note: left-upper corner image: SCPs, schwann precursor cells (blue); SCPs transition to an intermediate cell population called Bridge cells (red) as they transition to chromaffin cells (green) or Bridge cells (yellow) as they transition to Sympathoblasts (purple). The relative expression magnitude: blue low, white intermediate, and red high. C The mRNA levels of CASZ1 and CRC components in human normal adrenal gland (AG), neuroblastoma cell lines (NB_C) and neuroblastoma tumors (NB_T, Versteeg cohort) are analyzed using the R2:Genomics Analysis and Visualization platforms (gserver1.amc.nl/cgi-bin/r2/main.cgi). Data are presented as box and whisker plots with middle lines indicating medians and whiskers representing the 25th and 75th percentiles. The graph is generated using databases in the R2 platform.