Fig. 6: KLHL42 knockdown inhibited CTCL cell proliferation and promoted its apoptosis in vitro.
From: Single-cell analyses reveal novel molecular signatures and pathogenesis in cutaneous T cell lymphoma
A, B The KLHL42 mRNA and protein expression of PBMC CD4+ T cells in two HCs, two PE patients, two advanced-stage MF patients and one SS patient as well as HH and Hut78 cells were measured by qRT-PCR (A) and western blotting (B). C, D Suppression of KLHL42 mRNA and protein expression in Hut78 and HH cells by lentiviral transduction with shRNA sequences. E CCK-8 proliferation assays in Hut78 and HH cells with or without KLHL42 silencing. F Western blotting analysis of anti-apoptosis proteins in Hut78 and HH cells with or without KLHL42 silencing. G Long-term culture of the transduced cells revealed an increase in the Annexin V+PI- (early apoptosis) population in KLHL42-silenced cells compared with control cells. H, I 5-FU repressed GATA3 and KLHL42 expression in a dose-dependent manner. The mRNA and protein levels of both GATA3 and KLHL42 in Hut78 and HH cells were measured by qRT-PCR and western blotting. WT, wild type; NC, negative control. Data are presented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
