Fig. 1: Protein synthesis analysis in WT- and 3Tg-iAstro cells. | Cell Death & Disease

Fig. 1: Protein synthesis analysis in WT- and 3Tg-iAstro cells.

From: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

Fig. 1

a Cells were pulsed with puromycin (4 µM, 1.5 h), fixed and analysed by IF with anti-puromycin antibody (green) and with DAPI to stain nuclei (blue). Images were acquired with Leica Thunder imager 3D live cell microscope, scale bar = 25 µm. Data are expressed as mean ± SEM, WT-iAstro cells n = 40, 3Tg-iAstro cells n = 40, from 4 independent experiments; ****p < 0.05 by unpaired t-test. b WB with anti-puromycin antibody and ponceau staining on cells treated with 4 µM puromycin. Data are expressed as mean ± SEM of 12 independent experiments; ****p < 0.0001 by unpaired t-test.

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