Fig. 3: ER stress/UPR genes induction in WT- and 3Tg-iAstro cells.

Real-time PCR of Atf4 (a), Xbp1s (b) and Herpud1 (c) transcripts in cells treated or not with 1 µM THG for 4 h. Data of untreated WT- and 3Tg-iAstro cells (middle plots) and THG treated /untreated cell (right plots) are presented separately. Values represent mean ± SEM ∆C(t) of gene/S18 of four independent experiments for each condition. Left plots were analyzed using ANOVA, with Tukey posthoc test; middle and right plots were analyzed using unpaired two-tail Student’s t-test.