Fig. 4: Forced ER-mitochondria interaction causes impairment of protein synthesis in WT-iAstro cells. | Cell Death & Disease

Fig. 4: Forced ER-mitochondria interaction causes impairment of protein synthesis in WT-iAstro cells.

From: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

Fig. 4

WT-iAstro cells were non-transfected (WT Ctr) or transfected with mRFP (WT mRFP) and 10-nm ER- mitochondrial linker (WT 10 nm). a WB with anti-puromycin antibody and ponceau staining on lysates of cells treated with 4 µM puromycin (1.5 h); data are expressed as mean ± SEM of three independent experiments; ***p < 0.001 by one- way ANOVA, Sidak’s multiple comparison. b WB analysis of eIF2α phosphorylation. Data are expressed as mean ± SEM of four independent experiments; ****p < 0.0001 by one- way ANOVA, Sidak’s multiple comparison. c Cells pulsed with 4 µM puromycin (1.5 h) were fixed and analysed by IF with anti-puromycin antibody (green). Images were acquired with Zeiss 710 confocal laser scanning microscope, data are expressed as mean ± SEM of three independent coverslip; ***p < 0.001 by unpaired t-test. Scale bar = 25 μm. d Real-time PCR of Atf4, Xbp1s and Herpud1 transcripts in cells transfected with mRFP and 10-nm ER-mitochondrial linker; data are expressed as mean ± SEM of four independent wells.

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