Fig. 8: Proteomic analysis of WT- and 3Tg-iAstro secretome. | Cell Death & Disease

Fig. 8: Proteomic analysis of WT- and 3Tg-iAstro secretome.

From: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

Fig. 8

ACM (10 ml) was collected from 48 h culture of WT-iAstro, 3Tg-iAstro and 3Tg-iAstro cells treated with 4-PBA (3 μM, 48 h). Proteins were precipitated by TCA and processed as described in Methods section. 120, 84 and 102 proteins were identified in WT-iAstro, 3Tg-iAstro and 3Tg-iAstro + 4-PBA ACM, respectively. Protein quantification of 3Tg-iAstro vs WT-iAstro ACM returned five differentially regulated proteins (1.3 fold change cut-off, p < 0.05) (left upper table). Gene ontology analysis of proteins, unique for WT-iAstro or 3Tg-iAstro + 4-PBA both compared with 3Tg-iAstro ACM, returned GO terms related to extracellular matrix, focal adhesion and cadherin binding overrepresented in both WT-iAstro and 3Tg-iAstro + 4-PBA, but not in 3Tg-iAstro cells (right bottom table).

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