Fig. 3: GSDMD deficiency causes apoptosis in cardiomyocytes and exacerbates DIC. | Cell Death & Disease

Fig. 3: GSDMD deficiency causes apoptosis in cardiomyocytes and exacerbates DIC.

From: Gasdermin D mediates endoplasmic reticulum stress via FAM134B to regulate cardiomyocyte autophagy and apoptosis in doxorubicin-induced cardiotoxicity

Fig. 3

A, B GSDMD-FL, GSDMD-N, Cleaved-caspase 3 (Cleaved cas3) and BAX were measured using western blot assay, and relative band density was quantified. N = 3 per group. C, G Cell viability was determined using PI staining, and the live-to-dead cell ratios of GSDMD(flox/flox) and GSDMD-CKO cardiomyocytes were quantitated (scale bar: 100 μm). N = 6 per group. E, F The protein levels of GSDMD-FL, GSDMD-N, cleaved-caspase 3 (Cleaved cas3), and BAX in OE-C and GSDMD-OE cardiomyocytes treated with saline or DOX. N = 3 per group. D, H Cell viability was determined using PI staining, and the live-to-dead cell ratios of OE-C and GSDMD-OE cardiomyocytes were quantitated (scale bar: 100 μm). N = 6 per group. Data are depicted as the mean ± SEM. Statistical significance was determined by one-way and two-way ANOVA with a post hoc Holm–Sidak test, ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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