Fig. 6: ML-323 induces ER stress in human HCC cells. | Cell Death & Disease

Fig. 6: ML-323 induces ER stress in human HCC cells.

From: Inhibition of USP1 activates ER stress through Ubi-protein aggregation to induce autophagy and apoptosis in HCC

Fig. 6

A Flow cytometry analysis using an Annexin V-FITC/PI double-staining kit to detect apoptotic HCC cells. HCCLM3 and SMMC-7721 were cells treated with ML-323 alone or in combination with DMSO and an ER stress inhibitor (4-PBA). B Cell-counting kit-8 assay showing the viability of HCCLM3 and SMMC-7721 cells treated with ML-323 alone or in combination with DMSO and 4-PBA. Blocking ER stress alleviates the ML-323-induced inhibition of cell viability. C LC3B immunofluorescence staining in HCCLM3 and SMMC-7721 cells treated with ML-323 alone, or in combination with DMSO or 4-PBA. D LC3B puncta cells were statistically analyzed. E Western blot analysis of HCCLM3 and SMMC-7721 cells treated with ML-323 alone or in combination with DMSO or 4-PBA. Data are representative of at least three independent experiments (n = 3; error bars, SD). All data were representative of at least three independent experiments (n = 3; error bar, SD).

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