Fig. 6: The combination treatment induces pyroptosis through triggering cleavage of GSDME. | Cell Death & Disease

Fig. 6: The combination treatment induces pyroptosis through triggering cleavage of GSDME.

From: Targeted activation of GPER enhances the efficacy of venetoclax by boosting leukemic pyroptosis and CD8+ T cell immune function in acute myeloid leukemia

Fig. 6

A Western blot analysis of GSDME-F (full-length) and GSDME-N (N-terminal fragment) in the cell lines and primary blasts treated with G-1 and VEN alone or in combination for 24 h. B, C Western blotting of GSDME and GSDME-N in the cell lines infected with two independent sh RNA targeting GSDME, and then exposed to G-1 and VEN, alone or in combination for 24 h. D Representative light microscopy images of the leukemic cells (Scale bar: 50 μm). E LDH assay of LDH level in supernatants from cell cultures. F, G ELISA assay of IL-1β and IL-18 levels in supernatants from cell cultures. H CCK-8 assay of cell viability in the GSDME-silenced cells treated with the indicated drugs for 48 h. The data are expressed as the mean ± SD (n = 3). *p < 0.05, **p < 0.01; ***p < 0.001.

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